Check out the new publication in Photosynthesis Research, using the MultispeQ and PhotosynQ Platform (10.1007/s11120-017-0449-9)
Faster photosynthetic induction in tobacco by expressing cyanobacterial flavodiiron proteins in chloroplasts
Rodrigo Gómez, Néstor Carrillo, María P. Morelli, Suresh Tula, Fahimeh Shahinnia, Mohammad-Reza Hajirezaei, Anabella F. Lodeyro
Plants grown in the field experience sharp changes in irradiation due to shading effects caused by clouds, other leaves, etc. The excess of absorbed light energy is dissipated by a number of mechanisms including cyclic electron transport, photorespiration, and Mehler-type reactions. This protection is essential for survival but decreases photosynthetic efficiency. All phototrophs except angiosperms harbor flavodiiron proteins (Flvs) which relieve the excess of excitation energy on the photosynthetic electron transport chain by reducing oxygen directly to water. Introduction of cyanobacterial Flv1/Flv3 in tobacco chloroplasts resulted in transgenic plants that showed similar photosynthetic performance under steady-state illumination, but displayed faster recovery of various photosynthetic parameters, including electron transport and non-photochemical quenching during dark–light transitions. They also kept the electron transport chain in a more oxidized state and enhanced the proton motive force of dark-adapted leaves. The results indicate that, by acting as electron sinks during light transitions, Flvs contribute to increase photosynthesis protection and efficiency under changing environmental conditions as those found by plants in the field.
More PhotosynQ related publications are available here
On October 18, 2017, the Interdisciplinary Workshop on the dissemination of knowledge on “Intellectual Information Technologies in Education and Science” took place at the Faculty of Chemistry and Biology of the Ternopil National Pedagogical University (TNPU).
The co-organizers of this event were Andriy and Natalia Hertz, employees of the Department of General Biology and Methodology of Natural Sciences Teaching and the Department of Botany and Zoology (Faculty of Chemistry and Biology of TNPU).
According to the program, a demonstration of the possibilities of IT solutions in biological, educational and pedagogical research took place.
In particular, the on-line PhotosynQ platform was presented as a web tool for an integrated assessment of the physiological state of plants.
Information was disseminated on how the MultispeQ can measure, collect and analyze photosynthesis data in field and laboratory conditions.
The focus was on the openness and flexibility of the PhotosynQ platform and the development of educational tools through it, and more.
The students and faculty all wished to have the opportunity to work with MultispeQ and PhotosynQ and to evaluate the condition of plants for themselves.
Over the past several years there has been quite a bit of interest in measuring soil properties, which makes sense given that most of the plants we care about grow in soil. In response to that interest, we have developed numerous PhotosynQ prototypes, protocols and macros to measure soils over the past two years.
We started by using the MultispeQ to measure soil active C using potassium permanganate. This method used a cuvette to measure a color change in solution. The problem with using colorimetry, however, is that it requires users to do wet chemistry in the field. We would rather avoid that.
Another approach was to measure in situ C mineralization as an indicator of soil health. We have built multiple iterations of in situ soil C chambers (below). In general, the results from these chambers were positive, but there were a few drawbacks. One is that the results were highly dependent on soil moisture content and temperature. Therefore, we would need to collect a lot of data at different moisture and temperature conditions to account for these variations, much like we need to collect photosynthesis data at multiple light intensities to account for the effect of light on Phi2, PhiNPQ and PhiNO. The second problem is that the prototypes were quite clunky, and generated a lot of funny looks around campus when they were half-buried with random wires hanging out. If enough people were interested in using the in situ chambers, we could make a few mechanical changes to make them look less like an IED.
Going back to the drawing board, we brainstormed different ideas to simply assess soil health without having to take a lot of measurements or have multiple devices. This led us to develop a simple tool for measuring soil C mineralized from a sealed container. Using a “24-hour C mineralization burst” we can control for different temperature and moisture conditions by first air-drying, and then rewetting soil samples in quart jars. Then we use a syringe to sample headspace in the jar and inject it into a pass-through CO2 sensor.
The technology is pretty simple, just a CO2 sensor connected to a microcontroller, loaded with PhotosynQ firmware. To demonstrate our new SoilspeQ, we worked with a professor at MSU and took soil samples from a field where she was testing the effect of cover cropping on soil quality and maize productivity. We took many samples from different areas including soil that had mixed cover crops, soils without cover crops and some soil from the bare ground bordering the field plots. We also collected the soil from 1-5 cm and 5-10 cm deep, so we could see if there were differences between them. Check out the results here.
Our final approach to measuring soils is still in its early stages, but we are looking forward to see where it goes. We have teamed up with a professor at Colorado State University to develop microfluidics cards. The goal is to use reagent embedded cards to reduce in field wet chemistry and accurately measure key soil properties. We then use the MultispeQ or a version of the CoralspeQ to measure the color change. Our initial test, using Al3+ in solution at different concentrations is shown below.
Focusing on how the community is using PhotosynQ technologies. This month we are highlighting Sonya Lawrence, an Instructor in the Biological Sciences Program at MSU who has been incorporating PhotosynQ into her courses since 2014
As the first summer term came to an end, I headed over to North Kedzie Hall to check out the research posters being displayed by BS 172 students. This year marks three years that Sonya Michaud Lawrence, an instructor in Michigan State University’s Biological Sciences Program, has been using PhotosynQ as an educational tool in her lab classes.
Sonya was one of the first MultispeQ beta testers, beginning way back in the fall of 2014. Sonya uses PhotosynQ has a tool to help students learn the scientific method. Groups of students develop a hypothesis, design an experiment, use PhotosynQ to collect data and then use that data to test their hypothesis and learn statistical methods. Common research questions for her students include comparing how different species, canopy density, cardinal direction, time of day or proximity to fruit affects the photosynthetic efficiency of leaves.
Since Sonya’s first class project, her students have created more than 50 PhotosynQ projects and contributed tens of thousands of measurements. If you want to check out her students work, go the Discover tab on www.photosynq.org, scroll down to the Education section and select see all. Chances are those project were created by her students.
BS 172 student collecting PhotosynQ data on campus with the MultispeQ beta (left). Luke Weaver, Megan Campbell, and teammates present their findings at the BS 172 poster session (right).
As I meandered from poster to poster, talking to the students, a couple of themes kept popping up. Quite a few students mentioned that their results did not match their hypothesis. That’s ok! Happens in science all the time! Other students were impressed by how PhotosynQ made data collection easy, and by the amount of data they could collect in a short period of time. There were a few technical issues–with a couple old beta MultispeQ’s not working properly and measurements with the new MultispeQ’s taking too long because students were not familiar with the open-close start function. But overall, these students had a very different experience from those first brave students, back in the early beta testing days, when the software was still being developed and bugs frequently caused frustrations.
If you are wandering across MSU’s campus in the summer or fall and see students armed with MultispeQ’s and android phones, chances are they are Sonya’s students.
Following the PhotosynQ Workshop (see Dan’s post), we had moved to the LIL conference site at Laico Ouaga 2000, a high security hotel/conference venue outside of Ouagadougou city. “Feed the Future” is a program funded by USAID under the US government’s Global Hunger and Food Security Initiative. This program has been engaging many universities, institutions and private organizations in the US, Africa and Central/South America to improve the quality and management of legume, and contributing to the well-beings of local people. Michigan State University (http://legumelab.msu.edu/) is one of the leading institutions contributing researches and new technologies to the world.
One of the designated official languages being French, we had a simultaneous translation through headphone at this conference. The last time when I had to use French in daily basis was almost 20 years ago. Listening to the scientific talks was manageable, but my speaking ability was quite embarrassing. Another challenge was internet connectivity. As Dan mentioned, we had to manage the workshop with almost no internet connection. We were hoping to have a better connection at this best hotel in Burkina Faso, but unfortunately, it seemed the system could not handle a large traffic at once. The conference participants expressed that they had never experienced this in the past anywhere in Africa. It seems it was an isolated incidence, but we came up with some better solutions for the future.
PhotosynQ booth (From right: Dan, Frank and Atsuko)
Presentation by Dr. Irvin Widders, Director of Legume Innovation Lab, MSU. PhotosynQ was mentioned as one of the highlights of the ‘Feed the Future’ program.
At the last LIL conference held at Livingston, Zambia, Dave Kramer and Dan TerAvest presented the PhotosynQ project using MultispeQ Beta. This year in Burkina Faso, not only the people from Kramer Lab (Dave, Dan, Donghee Hoh, Isaac Osei-Bonsu and me), but also our PhotosynQ collaborators (Dr. Isaac Dramadri in Uganda, Dr. James Kelly with Dr. Jesse Traub and Dr. Wayne Loescher of MSU, and Dr. Kelvin Kamfwa of U of Zambia) presented more detailed and sophisticated data showing the correlations among photosynthesis, plant responses and gene expressions. It was very encouraging for us to see more people started thinking that the PhotosynQ platform and hand-held devices are useful and practical to the broad applications.
We are very excited about the new challenges, collaborations and long-lasting friendships. And we all hope to see you again!
On August 11 – 12, the PhotosynQ team conducted a workshop with researchers from across West Africa in Ouagadougou, Burkina Faso
The Kramer Lab has a cross-cutting grant from the McKnight Foundations Collaborative Crop Research Program, which provides resources for training and supporting local McKnight grantee’s throughout Western, Eastern, and Southern Africa. Using these resources, we were able to bring together members of the PhotosynQ team and 13 researchers from Niger, Mali and Burkina Faso for an intense, 2-day workshop. During the workshop, participants learned how to take MultispeQ measurements, create their own projects and interpret photosynthesis data. Additionally, and again with McKnight support, a number of participants were able to take MultispeQ instruments home from the conference, so that they can start their own PhotosynQ pilot projects.
It was one of the most challenging and fun workshops that I have participated in. On the one hand, the local researchers were very enthusiastic and ready to learn. There was a great mix of plant breeders, crop physiologists, and agronomists. This led to some lively discussion about how photosynthesis measurements, the MultispeQ and the PhotosynQ platform could all be integrated into local research projects, ultimately to the benefit of local smallholder farmers. Hopefully we can find ways to put some of the ideas generated into practice and see what happens!
On the other hand, internet connectivity was very poor, an obvious challenge for a web-based platform. Also, with all of the local researchers hailing from francophone countries, and with my French not extending beyond “bonjour,” the language barrier was a real hurdle. We did have translators who helped fill in the communication gaps, but they were not well versed in plant science lingo. They got a workout!
We look forward to long and productive collaborations with our new friends from West Africa!
We have developed a number of special features that can improve your PhotosynQ experience.
We have tried to build PhotosynQ to be flexible for a variety of different users, projects, goals, etc. Sometimes we have succeeded, sometimes we have not, and sometimes we have succeeded but failed to clearly explain the features that made it successful (which is not very helpful!). In fact, while writing this blog post I was reminded of a feature that we built, but then forgot about!
What kind of flexibility am I talking about?
Want to measure leaves that are too small to cover the light guides? You can do that!
Want to measure a lot of different plant populations without spending all day scrolling through long lists of multiple choice answers in the field? You can do that!
Want to read Barcodes and QR codes with the PhotosynQ apps? You can do that!
Want to upload custom data to the PhotosynQ database so you can compare it to your MultispeQ data? You guessed it, you can do that!